Real-time and online, with six different detection channels in multiplex experiments. Rely on the highly adaptable LightCycler® 480 System and related software, kits, disposables, reagents, and probes to perform gene expression analysis, SNP genotyping, and mutation scanning via high resolution melting (HRM). LightCycler® 480 Quick Guide Page i 5125-00-1113 General Information The contents of this document, including all graphics and photographs, are the property of Roche Diagnostics. Roche LightCycler 480 II Location: Weintraub Building, DE-550 Contact phone: (206) 667-6455 Contact fax: (206) 667-6845 Contact email: jcao@fredhutch.org Quantitative Real-Time PCR The LightCycler 480 from Roche is available for quantitative real-time PCR analysis and can perform methylation analysis and high-resolution melting analysis. When used with the LightCycler 480 System, this kit is ideally suited for hot start PCR applications. The LightCycler® 480 Instrument II is a rapid, plate-based, thermal block cycler with integrated real-time, online detection capabilities. LightCycler® 480 LED Lamp (430-630 nm) Detector Cooled monochrome CCD camera Filters Excitation (nm): 440, 465, 498, 533, 618 Detection (nm): 488, 510, 580, 610, 640, 660 Computer Pentium PC with Windows 7 4.4 55 15sec. This website contains information on products which is targeted to a wide range of audiences and could contain product details or information otherwise not accessible or valid in your country. 4.4 ↓ 95 1min. 2.2 ↓ 95 15sec. The LightCycler® 480 System features a versatile, plate-based, real-time PCR device that supports mono- or multicolor applications. Please disable any ad blockers and view the site in non-private mode. The LightCycler® 480 Instrument II is a rapid, plate-based, thermal block cycler with integrated real-time, online detection capabilities. The LightCycler® 480 System is a high-performance, medium- to high-throughput PCR platform (96- or 384-well plates) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. Obtain fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids and SNP analysis with the LightCycler® 96 System. Roche LightCycler® 480システムII を使用する場合 機器の設定は以下の通り 【サイクル条件】 Ramp Rate( /sec) 50 10min. Roche Applied Science LightCycler® 480 Real-Time PCR System Rapid by nature, accurate by design Planned introduction of the LightCycler® 480 System: September 2005 2 Combining amazing speed with exceptional accuracy Item LightCycler®480 Instrument II Company Roche Price Get Quote Get Quote for this Product Catalog Number 5015278001 / 5015243001 Quantity EA Applications Gene detection / Gene expression analysis / And genetic The LightCycler® 480 System is a high-performance, medium- to high-throughput PCR platform (96- or 384-well plates) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array Access package inserts through your country’s Roche Diagnostics Website. No part may be reproduced or transmitted in any Based on a comprehensive improvement of the Peltier-based technology incorporated in a silver block, the instrument provides extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility. Each pack of LightCycler ® 480 Multiwell Plates 96 contains 5 x 10 plates in five re-sealable, non-sterile plastic bags. In combination with the LightCycler®480 System and suitable PCR primers, this kit allows very sensitive detection and quantification of defined DNA sequences. The Lightcycler 480 can be reserved via the electronic reservations system. プレートベースのLightCycler®480 System II は、遺伝子発現および遺伝子変異解析 に対して、適応性および汎用性が高いリアルタイムPCR システムです。 ­ÓÔ#FÊ6¼ÏõKhìÅe#ÃÝcþ‹F×Úi‚íŒ+oö…‰rB½7¬Ï°¿Ô§×©{ŒÔÝw¬[sV“늇~¶çAmÔ:.q(=ÐÂ-B"P½8iØÄ-{äþ9 hÉ1Û«#ь7ZK „µvÝ´\Ö´ 1e=×WÊ®Q¬WêL®AlÂò®C?Â!Û7ß®»?áï6á¿ñ(Ÿ±Ä.W’$s&l‡½)Ágî;ëVu‹™\?÷À?‡°‡)LXpPߊ ŽÀ°î]÷™±Î¾sAbD÷¿HEfÄ^}2)ë½ã‘;φ+uIКPòÀ¶°ˆ£1Žˆ÷’¸â²\T•à^ÀŽÈ©hàÜ(ÄJíé2+‡Wo_f¶µ4©=_JIfˆŒFé´#šš! Biomedical scientists, academics and others engaged in medical research, Laboratory managers, Doctors, Nurses and others directly related to provision of health care services, Patients, caregivers and others not directly involved in the provision of health care services. Some randomr randomr randomr randomr randomr randomr, More detailed information about the LightCycler, cobas p 512 and cobas p 612 pre-analytical systems, cobas p 501 / p 701 post-analytical units, uPath PD-L1 (SP263) image analysis, NSCLC (CE-IVD), Relative quantification/gene expression analysis, Melt-curve genotyping with HybProbe- or SimpleProbe probes, Endpoint genotyping with hydrolysis probes, Gene Scanning or Methylation analysis with High Resolution Melting (HRM), Fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids, mutation scanning, and SNP analysis, Basic and advanced methods for gene expression and genotyping based on endpoint analysis or melting curves, Wide range of probe formats and fluorescent dyes, White or clear multiwell plates to best fit your assay format, application, and workflow, Flexibly switch between plate formats and thermal block cyclers in just a few minutes with no recalibration time, Introduce automated pipetting and plate loading and integrate into your lab information management system (LIMS), Build a modular real-time PCR platform to meet your particular needs. How to Use this Product y Version: 13 Two-step RT-PCR LightCycler® 480 SYBR Green I Master can also be used to perform two-step RT-PCR. Please be aware that we do not take any responsibility for accessing such information which may not comply with any legal process, regulation, registration or usage in the country of your origin. Since LightCycler® 480 Software does #) LightCycler® 480 Software is designed to provide compatibility#) with FDA’s 21 CFR Part 11 requirements. LightCycler Multiplex RNA Virus Master Mix on the Roche LightCycler 480 II* TaqPath1-Step Multiplex Master Mix: Sensitivity ⊿Ave Cq of 50ng (TaqPath vs. LightCycler) 5 10 15 20 25 30 35 40 LightCycler Multiplex RNA Use the project number and name when entering reservations and only reserve the exact timeslots needed. For life science research only. Login with the nethz login and password. Benefit from cutting-edge thermal block and optical technology that leads to excellent well-to-well homogeneity and maximized detection sensitivity. LightCycler®480 II实时荧光定量PCR仪采用了宽光谱、高强度的白色固态光源，其发射波长范围宽泛覆盖整个可见光 区域，激发光滤镜与发射光滤镜任意组合可获得多达20种检测模式，能够使用所有定量PCR和高分辨率熔解曲线研究的荧光染料。 Request a Quote The LightCycler® 480 Real-Time PCR System Roche Applied Science brings you one more breakthrough in the real-time PCR analysis of gene expression and genetic variation Roche Applied Science’s cutting-edge gene analysis technology is now available in a high-throughput, microwell plate-based cycler format (96- or 384-well). You appear to be using incognito/private browsing mode or an ad blocker, which may adversely affect your experience on the site. Roche knows real-time PCR. The benchtop instrument is easily customizable to meet changing user requirements, and can be integrated into everyday use as an automated high-throughput solution. LightCycler­® 480 Instrument II. Sealing foils are supplied in a … Roche LightCycler Whether your interest is in gene expression profiling or in detecting genetic variations, there is a member of the LightCycler® System family offering the analytical performance and throughput you need for your research. Amplify your success in medium- and high-throughput real-time PCR applications. この先進的なテクノロジーをさらに進化させたLightCycler® 480 System II の、精巧にデザインされたサーマルブロック、光学システムおよびソフトウェアにより、至高の高感度、正確性、再現性を実現します。 Login with the nethz login and password. Shipping and handling. The LightCycler ® 480 High Resolution Melting Master can be used in conjunction with Uracil DNA Glycosylase for carryover prevention during PCR. El sistema LightCycler ® 480 es una plataforma de PCR de alto rendimiento y de capacidad media a alta que proporciona diferentes métodos para la detección génica, el análisis de expresión génica, análisis de variación genética y validación de datos de matrices. The benchtop instrument is easily customizable to meet changing user requirements, and can be integrated into everyday use as an automated high-throughput solution. Monitor amplification of 32 PCR products simultaneously with the LightCycler® 2.0 System. Roche LightCycler 480 real-time PCR run setup instructions for RT2 Profiler PCR Arrays LightCycler®480 SYBR Green I Master is designed for research studies. Not for use in diagnostic procedures. The ideal combination of speed, accuracy and versatility. Furthermore, the LightCycler ® 480 Software, Version 1.5 enables different analysis capabilities for product characterization (SYBR Green I assays, T m calling), … The software is compatible with the regulations for electronic records (Subpart B). The LightCycler ® 480 System features a versatile, plate-based, real-time PCR device that supports mono- or multicolor applications. The LightCycler ® 480 System is a high-performance, medium- to high-throughput PCR platform (96- or 384-well plates) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. 2 Go forward, confidently. LightCycler ® 480 Probes Master is a ready-to-use reaction mix specifically developed for the hydrolysis probe detection format in multiwell plates on the LightCycler ® 480 Instrument. Le système LightCycler ® 480 est une plateforme pour la PCR avec une performance élevée prouvée pour les débits d’échantillons moyens à élevés qui offre diverses méthodes pour la détection de gènes, l’analyse de l’expression de gènes, l’analyse des variations génétiques et la validation des données de puces à ADN. Roche LightCycler 480 II 96 real-time PCR system. The LightCycler ® 480 System is characterized by providing highly reproducible PCR and melting curve analysis conditions across the assay plate, leading to highly discriminative analytical capabilities (e.g., when distinguishing 1000 from 2000 target copy numbers) and very low intra-well, intra-assay variation even on the edges of multiwell plates. The LightCycler® 480 System is a proven high-performance, medium-to high-throughput PCR platform that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. The LightCycler® 480 Instrument II 96- to 384-well throughput, versatile for experienced users The LightCycler® 480 Instrument is a high-performance, flexible throughput qPCR platform (with interchangeable 96- or 384-well blocks) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and pathogen detection. Das Tischgerät lässt sich ohne Weiteres an veränderte Anforderungen in der Praxis anpassen und als automatisierte Lösung für hohen Durchsatz in den Routinegebrauch integrieren. Check the Roche website for more details. ®LightCycler 480 SYBR Green I Master 6 lifescience.roche.com 2. ロシュは、COVID-19パンデミックに終止符を打つために全世界で行われている活動を全力で支援しています。世界中で、私たちは、医療システムと患者さんにとって必要不可欠な高品質の検査を提供するために、日々、スピード感・熱意・目的意識を持って取り組んでいます。 The LightCycler ® 480 Software, Version 1.5 provides all functionalities for running real-time PCR applications, such as user-friendly programming of PCR protocols, sample editing, templates, and macros. To import your sample names into the Lightcycler software use the plate maker file: Lightcycler_platemaker_384 (XLSX, 41 KB) Lightcycler_platemaker_96 (XLSX, 15 KB) Reservation. Roche社のリアルタイムPCR装置LightCycler 480用の96ウェルまたは384ウェルタイプのマイクロプレートです。白色タイプと透明タイプがあります。 ※ 本製品は研究用です。研究用以外には使用できません。 Based on a comprehensive improvement of Peltier-based technology incorporated in a silver block, the instrument provides extraordinary well-to-well temperature homogeneity and inter-well, inter-cycle reproducibility. Generate meaningful results via the state-of-the-art data analysis methods, in line with MIQE guidelines, and analyze true raw data without the need for passive reference dyes or normalization plates. LightCycler® 480 Quick Guide Page i 5125-00-1113 General Information The contents of this document, including all graphics and photographs, are the property of Roche Diagnostics. Downloads. Our renowned LightCycler® 480 System represents the We just picked it up from the lab and they are trying to find the missing side panels but are thinking they have been thrown away. 2.2 60 45sec. Rapid high-throughput, plate-based real-time PCR amplification and detection instrument. In two-step RT-PCR, the reverse transcription of RNA into cDNA is separated from the other reaction steps and is performed outside the Seller assumes all responsibility for this listing. The Lightcycler 480 can be reserved via the electronic reservations system. LightCycler® 96は、リアルタイムPCRのスタンダードとして高い評価をいただいているLightCycler® 480と同等の性能を有していながら、お求めやすい価格も両立しています。 特長 迅速なPCR反応（40サイクル＜40分～60分） PC不要で Achieve consistent, high quality performance with a master mix containing ResoLight, a novel dye that does not inhibit amplification enzymes or interfere with PCR when added during reaction setup. ライトサイクラー480 インスツルメント96ウェルタイプの優れた温度制御と光学特性に完全にフィットする96ウェルプレートです。 The LightCycler® 480 Instrument II is a rapid, plate-based, thermal block cycler with integrated real-time, online detection capabilities. Das LightCycler ® 480 System besteht aus einem vielseitigen, plattenbasierten Echtzeit-PCR-Gerät, das Single- oder Multicolour-Anwendungen unterstützt. For more than 15 years, we’ve been innovating instrumentation and reagents to bring the best technologies to your lab. We believe it's urgent to deliver medical solutions right now - even as we develop innovations for the future. {ÖŶ(/%§‚p†—Bvù¼@áDNë[3³uŽdâû㘇AVbL¡þe¯¢ûÉ˽H­:oÆH© à]$­P:¡©ß\ˆx»±Zž"uFoõð%zÁÆßu?,iê'+„d‹iŸ£D i|Ì!’ô«ÞãükÎwŽx²bK>îXgQ^,çEd.
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